Figure 2.

Lipopolysaccharide (LPS) does not induce hepatocyte death in vitro or in vivo. (A) Immunofluorescence of liver from wild-type (WT) mice at 4 h after intraperitoneal injection with LPS (5 mg/kg). TMR = red; 4′,6-diamidino-2-phenylindole (DAPI) = blue; actin = white. (B) Plasma alanine aminotransferase (ALT) level in WT mice at 4 h after intraperitoneally injection with LPS (5 mg/kg). Each point represents one mouse. (C) WT hepatocytes were treated with LPS (1 μg/ml) for 24 h. Cytotoxicity was measured by using lactate dehydrogenase (LDH) release in the culture media. Data are expressed as mean ± SEM. (D) Immunofluorescence of Zombie-red staining (cell death) of WT hepatocytes 24 h after treatment with LPS (1 μg/ml). DAPI = blue. NS, no significant difference. n = 3.