Figure 5.

T cells stimulated by peptides+HB_100-108/pulsed DCs exert considerable lysis of the PANC-1 cell line in vitro. After stimulation with empty DCs or peptides±HB_100-108/pulsed DCs for 24 hours or no stimulation, allogeneic T cells were collected as the effector cells and the PANC-1 cancer cell line was used as target cells. The target cells were cocultured with the effector cells at a T : E ratio of 1 : 5. The cytolytic activity was assessed by measurement of lactate dehydrogenase (LDH) release. This experiment has been performed in triplicate and repeated three times with similar results.