Figure 10.

PEITC suppressed OS growth in vivo. MNNG/HOS cells were injected subcutaneously into the right flank of the male BALB/c nude mice. One week after the OS xenograft mouse model was established, the mice were randomly divided into two groups and, respectively, administrated with 10% sesame seed oil and 30 mg/kg PEITC once daily for 24 consecutive days. (a) Body weight change of the two groups. (b) Volume change of OS tissues of the two groups. Data were calculated by the formula: volume = length × width² × 0.5. (c) Weight of OS tissues from the two groups. (d) H&E staining analysis of tumor tissues (200× and 400×). (e) The protein expression levels of TfR1, DMT1, FTH1, and FPN in tumor tissues. (f) The protein expression levels of LC3B, C-caspase3, GPx4, and Cdc2 in tumor tissues. (g) Phosphorylation levels of ERK, p38, and JNK in tumor tissues. All data were presented as the means ± SD (n = 7). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 versus control group.