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. 2020 Apr 4;2020:5021983. doi: 10.1155/2020/5021983

Figure 5.

Figure 5

PEITC induced oxidative stress in human OS cells. (a) ROS level in MNNG/HOS, U-2 OS, MG-63, and 143B cells treated with the indicated concentrations of PEITC for 24 h by using a DCFH-DA sensor. (b) Lipid peroxidation level in MNNG/HOS, U-2 OS, MG-63, and 143B cells treated with the indicated concentrations of PEITC for 24 h by using a BODIPY 581/591 C11 sensor. (c) Quantitative analysis of lipid peroxidation in (b). (d) MDA level in MNNG/HOS, U-2 OS, MG-63, and 143B cells treated with the indicated concentrations of PEITC for 24 h. (e) GSH/GSSG ratio in MNNG/HOS, U-2 OS, MG-63, and 143B cells treated with the indicated concentrations of PEITC for 24 h. (f) GPx4 expression in MNNG/HOS, U-2 OS, MG-63, and 143B cells treated with the indicated concentrations of PEITC for 20 h or 30 μM PEITC for 4 h, 12 h, 24 h, and 48 h. All data were presented as the mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus control group.