Table 2.
Summary of the bacterial reverse mutation test, confirmatory mutation test.
| Confirmatory mutation test (preincubation test) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Concentrations (μg/plate) | Salmonella typhimurium tester strains | Escherichia coli | ||||||||||||||||||
| TA 98 | TA 100 | TA 1535 | TA 1537 | WP2 uvrA | ||||||||||||||||
| −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | |||||||||||
| Mean values of revertants per plate mutation rate (MR) | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR |
|
| ||||||||||||||||||||
| Untreated control | 19.0 | 0.86 | 32.3 | 1.01 | 93.7 | 1.16 | 112.0 | 1.10 | 9.7 | 1.00 | 11.7 | 0.85 | 7.7 | 0.96 | 7.7 | 0.70 | 29.3 | 0.73 | 41.7 | 1.08 |
| DMSO control | 21.7 | 1.00 | 30.7 | 1.00 | — | — | 91.7 | 1.00 | — | — | 10.3 | 1.00 | 7.3 | 1.00 | 6.7 | 1.00 | — | — | 39.7 | 1.00 |
| Ultrapure water control (100 μL) | — | — | — | — | 92.0 | 1.00 | — | — | 9.3 | 1.00 | — | — | — | — | — | — | 30.7 | 1.00 | — | — |
| Ultrapure water control (250 μL) | 22.0 | 1.00 | 32.0 | 1.00 | 81.0 | 1.00 | 102.0 | 1.00 | 9.7 | 1.00 | 13.7 | 1.00 | 8.0 | 1.00 | 11.0 | 1.00 | 40.3 | 1.00 | 38.7 | 1.00 |
|
| ||||||||||||||||||||
| 5000 | 27.0 | 1.23 | 30.7 | 0.96 | 92.7 | 1.14 | 104.0 | 1.02 | 12.0 | 1.24 | 15.3 | 1.12 | 6.7 | 0.83 | 8.7 | 0.79 | 40.3 | 1.00 | 64.7 | 1.67 |
| 1600 | 27.7 | 1.26 | 31.0 | 0.97 | 86.3 | 1.07 | 93.0 | 0.91 | 9.3 | 0.97 | 14.7 | 1.07 | 7.7 | 0.96 | 9.7 | 0.88 | 32.3 | 0.80 | 50.7 | 1.31 |
| 500 | 29.7 | 1.35 | 30.7 | 0.96 | 83.7 | 1.03 | 94.0 | 0.92 | 13.7 | 1.41 | 10.3 | 0.76 | 7.0 | 0.88 | 9.0 | 0.82 | 33.7 | 0.83 | 49.3 | 1.28 |
| 160 | 28.0 | 1.27 | 19.3 | 0.60 | 84.3 | 1.04 | 97.7 | 0.96 | 10.0 | 1.03 | 12.3 | 0.90 | 7.0 | 0.88 | 10.3 | 0.94 | 33.0 | 0.82 | 55.0 | 1.42 |
| 50 | 23.0 | 1.05 | 24.3 | 0.76 | 86.0 | 1.06 | 102.3 | 1.00 | 9.3 | 0.97 | 14.3 | 1.05 | 9.7 | 1.21 | 11.3 | 1.03 | 33.7 | 0.83 | 46.7 | 1.21 |
| 16 | 21.0 | 0.95 | 27.7 | 0.86 | 94.7 | 1.17 | 104.3 | 1.02 | 7.7 | 0.79 | 11.3 | 0.83 | 8.0 | 1.00 | 8.7 | 0.79 | 37.7 | 0.93 | 41.3 | 1.07 |
|
| ||||||||||||||||||||
| NPD (4 μg) | 386.7 | 17.85 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| SAZ (2 μg) | — | — | — | — | 981.3 | 10.67 | – | – | 1053.3 | 112.86 | — | — | — | — | — | — | — | — | — | — |
| 9AA (50 μg) | — | — | — | — | — | — | — | — | — | — | — | — | 570.0 | 77.73 | — | — | — | — | — | — |
| MMS (2 μL) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 936.0 | 30.52 | — | — |
| 2AA (2 μg) | — | — | 1018.7 | 33.22 | — | — | 2154.7 | 23.51 | — | — | 149.0 | 14.42 | — | — | 149.7 | 22.45 | — | — | — | — |
| 2AA (50 μg) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 170.0 | 4.29 |
Abbreviations: 2AA: 2-aminoanthracene; 9AA: 9-Aminoacridine; DMSO, dimethyl sulfoxide; MMS: Methyl-methanesulfonate; MR: Mutation Rate; NPD: 4-Nitro-1,2-phenylenediamine; S-9, metabolic activation; SAZ: sodium azide; ∗DMSO was the vehicle of the positive control substances: NPD, 9AA and 2AA; ultrapure water was the vehicle of the test item and for SAZ and MMS. The mutation rates of the test item, untreated, and positive controls were calculated using the data from their respective vehicle controls.