Table 2.
The primer sequences used in the real-time PCR.
| Genesa | Sequences (5′-3′)b | Gene bank no. |
|---|---|---|
| MyD88 | F: ATGGTGGTGGTTGTCTCTGAC R: GGAACTCTTTCTTCATTGGCTTGT |
GQ221044 |
| TLR-2 | F: CAAGAGGAAGCCCAGGAAG R: TGGACCATGAGGTTCTCCA |
DQ890157 |
| TLR-4 | F: TGCTGGCTGCAAAAAGTATG R: CCCTGTAGTGAAGGCAGAGC |
HQ343416 |
| TLR-9 | F: ATGGGCCCCTACTGTG R: CTATTCGGCTGTCGTGG |
HQ263217.1 |
| TRAF-6 | F: TCAGAGAACAGATGCCCAAT R: GCGTGCCAAGTGATTCCT |
XM_012134166.2 |
| IL-6 | F: AGGAAAAAGATGGATGCTTCCA R: GACCAGCAGTGGTTTTGATCAA |
NM_001009392 |
| IL-1β | F: CGTCTTCCTGGGACGTTTTAG R: CTGCGTATGGCTTCTTTAGGG |
NM_001009465 |
| NF-κB | F: ATACGTCGGCCGTGTCTAT R: GGAACTGTGATCCGTGTAG |
XM_005226864.2 |
| TNF-α | F: ACACCATGAGCACCAAAAGC R: AGGCACAAGCAACTTCTGGA |
NM_001024860.1 |
| Fas | F: TTTTGCTGTCAGCCTTGTCC R: TGTTCCACTTCTAGCCCATG |
NM_001123003.1 |
| Fasl | F: TCTGTGGAGAAGCAAATAGGTC R: AGGGCAATTCCATAGGTGTC |
XM_004013705.1 |
| P53 | F: CCCGCCTCAGCACCTTAT R: GCACAAACACGCACCTCA |
NM_001009403.1 |
| Caspase-3 | F: GCTCGAGCTCATGCACATTC R: CCATTGGGCACTTGGCATAC |
NM_001286089.1 |
| Caspase-8 | F: TTAGCATAGCACGGGAGCAG R: GTCAGCTCATAGATGGGGGC |
XM_005676365.1 |
| Caspase-9 | F: AGTCAGGCCCTTCCTTTGTT R: ATGGGTCCTGCTTCATCACT |
XM_005690814 |
| Bcl-2 | F: CGAGTGGCGGCTGAAAT R: GGTCTGCCATGTGGGTGTC |
HM630309.1 |
| Bax | F: ATGGGCTGGACATTGGACTT R: ACTGTCTGCCATGTGGGTGT |
AF163774.1 |
| β-Actin | F: GCTCTTCCAGCCGTCCTT R: TGAAGGTGGTCTCGTGAATGC |
NM_001009784.1 |
aMyD88: myeloid differentiation factor 88; TRAF-6: TNF receptor-associated factor 6; TLR: Toll-like receptor; IL: interleukin; NF-κB: nuclear factor kappa-B; TNF-α: tumor necrosis factor alpha; Bax: Bcl-2-associated X protein; Bcl-2: B-cell lymphoma/leukaemia 2; p53: protein 53; Fas: apoptosis antigen 1; Fasl: Fas ligand. bF: forward; R: reverse.