Figure 3.

Neuronal differentiation of recovered AHPCs after 4 days of encapsulation. Fluorescence images of AHPCs illustrating immunoreactivity for TuJ1 24 h after recovery (A₁–C₁) and 72 h after recovery (A₂–C₂): TuJ1-Cy3 (red) with DAPI staining (blue). AHPCs cultured in MM (A₁,A₂), alginate control (B₁,B₂), and recovered from hydrogels (C₁,C₂). Asterisks indicate the location of higher magnification inset images. Higher magnification images are of the TuJ1-Cy3 channel only. Scale bar = 50 μm (20 μm for insets). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole. (D₁,D₂), Quantitative analysis of AHPCs immunoreactive for the TuJ1 antibody. Error bars represent standard error of the mean. N = 6 independent experiments, 5 imaging fields per experiment. *Significantly different at p ≤ 0.05.