Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 9;11:621. doi: 10.3389/fimmu.2020.00621

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Tanaka, Nagai, Okumura, Greene and Kambayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PRMT5 is required for the proliferation of naïve and effector/memory T cells in a development-independent manner. FACS-sorted CTV-labeled naïve and effector/memory CD8⁺ and CD4⁺ T cells from tamoxifen-treated Cre-ERT2 and Cre-ERT2 PRMT5^fl/fl mice were stimulated for 3 d with anti-CD3 and anti-CD28-coated beads in the presence of IL-2, and analyzed by flow cytometry. (A) The frequency of live cells (Live/Dead near-IR^–) among CD8⁺ T cells (CD90.2⁺ CD8a⁺) and CD4⁺ T cells (CD90.2⁺ CD4⁺) is plotted as mean ± SEM. (B) The counts of live T cells are normalized to the initial (day 0) counts and plotted as mean ± SEM. (C) Representative plots show the dilution of CTV and expression of PRMT5. The data are representative of two independent experiments. **P < 0.01, ***P < 0.001, by unpaired two-tailed Student’s t-test. NS, not significant.