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. 2020 Apr 15;20:96. doi: 10.1186/s12866-020-01781-z

Table 5.

Specificity results of the PCR assay

Primer name Detected species Ct value Tm (°C)
Gasseri-F,R L. gasseri KCTC 3163 5.366 83.749
Rhamnosus-F,R L. rhamnosus KCTC 3237 11.258 79.329
Brevis-F,R L. brevis KCTC 3498 5.762 85.319
Sakei-F,R L. sakei KCTC 3603 11.139 82.441
Johnsonii-F,R L. johnsonii KCTC 3801 6.450 84.193
Jensenii-F,R L. jensenii KCTC 5194 6.583 81.378
Fermentum-F,R L. fermentum KACC 11441 4.260 88.582
Plantarum-F,R L. plantarum KACC 11451 10.715 82.027
Paracasei-F,R L. paracasei KACC 12361 12.012 80.746
Paraplantarum-F,R L. paraplantarum KACC 12373 10.884 82.306
Casei-F,R L. casei KACC 12413 10.739 82.513
Curvatus-F,R L. curvatus KACC 12415 13.832 82.686
Acidophilus-F,R L. acidophilus KACC 12419 12.383 79.308
Salivarius-F,R L. salivarius KCTC 3600 14.905 81.806
Reuteri-F,R L. reuteri KCTC 3594 9.142 83.439
Coryniformis-F,R L. coryniformis KACC 12411 13.638 84.793
Farciminis-F,R L. farciminis KACC 12423 10.678 80.465
Zymae-F,R L. zymae KACC 16349 7.546 82.568
Pentosus-F,R L. pentosus KACC 12428 11.603 84.268
Crustorum-F,R L. crustorum KACC 16344 12.467 82.012
Mucosae-F,R L. mucosae KACC 12381 11.598 83.109
Buchneri-F,R L. buchneri KACC 12416 11.606 82.206
Helveticus-F,R L. helveticus KACC 12418 12.087 79.059
Amylovorus-F,R L. amylovorus KACC 12435 11.256 82.037
Heilongjiangensis-F,R L. heilongjiangensis KACC 18741 11.922 81.205
Parabuchneri-F,R L. parabuchneri KACC 12363 9.377 81.604
Acidipiscis-F,R L. acidipiscis KACC 12394 10.743 81.566
Sanfranciscensis-F,R L. sanfranciscensis KACC 12431 10.273 79.814
Ruminis-F,R L. ruminis KACC 12429 9.724 82.341
Agilis-F,R L. agilis KACC 12433 11.758 82.095
Delbrueckii-F,R L. delbrueckii KACC 12420 8.621 83.114
Amylophilus-F,R L. amylophilus KACC 11430 10.943 82.733
Kunkeei-F,R L. kunkeei KACC 19371 8.542 83.217
Acetotolerans-F,R L. acetotolerans KACC 12447 11.912 82.031
Lindneri-F,R L. lindneri KACC 12445 12.910 79.917
Gallinarum-F,R L. gallinarum KACC 12370 10.132 78.138
Amylolyticus-F,R L. amylolyticus KACC 12374 11.694 83.460