Table 3.
Sensitivity of the TK1 antibodies
| Clone | ID | Isotype | Host | Mean | SD± | Inter CV | Intra CV |
|---|---|---|---|---|---|---|---|
| LOD pg/ml | LOD | % | % | ||||
| 4G10 | 1-1 | IgG2a, κ | Ms | 146.40 | 33.96 | 16.4 | 5.72 |
| *10E8 | 1-2 | IgG1, κ | Ms | 44.67 | 4.33 | 9.69 | 11.40 |
| *7H2 | 1-7 | IgG1, κ | Ms | 19.79 | 0.55 | 2.78 | 9.71 |
| 7D1 | 1-9 | IgG1, κ | Ms | 94.03 | 12.86 | 13.68 | 8.41 |
| *1B12 | 2-1 | IgG2a, κ | Ms | 38.76 | 4.84 | 12.5 | 4.57 |
| 3G7 | 2-6 | IgG2a, κ | Ms | 125.96 | 21.0 | 16.67 | 1.09 |
| 10H2 | 2-15 | IgG2a, κ | Ms | 56.50 | 15.96 | 20.3 | 8.2 |
| *8G2 | 2-17 | IgG2b, λ | Ms | 72.87 | 4.83 | 1.62 | 8.38 |
| *9C10 | 2-19 | IgG2a, κ | Ms | 11.01 | 0.67 | 6.1 | 4.0 |
| *3B4 | 3-5 | IgG2b, κ | Ms | 66.90 | 3.5 | 5.33 | 0.45 |
| 6A11 | 3-6 | IgG1, κ | Ms | NA | NA | NA | NA |
| 8F12 | 3-10 | IgG1, κ | Ms | 101.00 | 16 | 15.84 | 2.5 |
| 5F7G11 | 4-2 | IgG2a, λ | Rat | 1376.58 | 153.42 | 11.14 | 0.71 |
| *2E8 | 5-1 | IgG2b, λ | Ms | 33.10 | 0.50 | 1.52 | 1.5 |
| 9A9 | 5-10 | IgG2b, κ | Ms | 12,006.28 | 244.36 | 2.04 | 1.76 |
| *3B2E11 | 6-1 | IgG2a, λ | Rat | 10.73 | 1.85 | 17.32 | 6.58 |
| 1D3G4 | 6-2 | IgG2a, λ | Rat | NA | NA | NA | NA |
| EPR3194 | Abcam | Unknown | Rb | 16.48 | 1.045 | 6.33 | 10.2 |
| 91651 |
The LOD values are expressed as mean values from two independent immunoassays. Precision and uncertainty are reflected in the coefficient of variance between assays. The most sensitive antibodies are marked with stars