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. 2020 Apr 15;8:49. doi: 10.1186/s40478-020-00920-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Detection of AQP4-specific antibodies in sera of Lewis rats immunized with mimotopes. Life-cell immunofluorescence staining against the surface antigens rat AQP4-EmGFP and human AQP4-EmGFP was made. Antigens were transiently transfected into HEK293 cells (green) and sera of Lewis rats immunized with mimotopes IV-04, III-01, I-13, I-04, or IV-38 were added. Bound antibodies were then detected with anti-rat-IgG (red). Merge of green and red signals reveals surface staining of transfected cells in yellow if antibody signals were strong. Additional images at 40x magnification were added for details (see inlay). For titer values see Table 3