Fig. 6.

Validation of TMED2 and TMED10 expression in CDR1as CRISPRi cell lines. a Generation of CDR1as knockout cell lines using CRISPR/Cas9. A map of lentiCRISPR V2 vector and CDR1as deletion sites is shown. b CDR1as expression in CRISPRi cell lines. c Cell proliferation of CDR1as CRISPRi 293 T cells relative to WT cells. d TMED2 and TMED10 expression in CDR1as CRISPRi 293 T or WT cells as determined by western blotting (cropping of blots, full-length blots are presented in Fig. S6). e A xenograft assay conducted in nude mice using CDR1as CRISPRi 293 T cells. Tumor size and tumor weight are shown. f Tumor volumes from mice in (e). g Tumor CDR1as, TMED2, and TMED10 expression as determined via qRT-PCR. h Measurements of TMED2 and TMED10 via western blotting (cropping of blots, full-length blots are presented in Fig. S7)