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Primers used for reverse transcription and PCR in this study
| Gene | Primer sequence (5′–3′) |
|---|---|
| RUNX2 | F: CTCTTCCCAAAGCCAGAGTC |
| R:CAGCGTCAACACCATCATTC | |
| OSX | F: GAGGAAGAAGCCCATTCACA |
| R:GCAGGCAGGTGAACTTCTTC | |
| ALP | F: AACCCAGACACAAGCATTCC |
| R:GCCTTTGAGGTTTTGGGTCA | |
| OPN | F:GACGATGATGACGATGG |
| R:CCTCAGTCCATAAGCCAAGC | |
| GADPH | F:AAGGTCATCCCAGAGCTGAA |
| R:AGGAGACAACCTGGTCCTCA | |
| U6 | F:ATTGGAACGATACAGAGAAGATT |
| R:AGGAACGCTTCACGAATTTG | |
| miR-505 | F:GCGGGAGCCAGGAAGTAT |
| R:CAGTGCGTGTCGTGGAGT |
