Table 1.

Primers used in real-time RT–PCR analyses

Genes	Forward sequence	Reverse sequence	Product size (bp)	Annealing temp.(°C)
Actb	AAGTACCCCATTGAACACGG	ATCACAATGCCAGTGGTACG	257	52
B2m	GGACTGGTCTTTCTATATCCTGGC	GATCACATGTCTCGATCCCAGTAG	150	58
Cldn11	TGTCAACAGCAGCAAGATGGCG	GCTCCAAGGGCCTGTGGGC	332	57
Dll1	TCAGATAACCCTGACGGAGGC	AGGTAAGAGTTGCCGAGGTCC	185	56
Dll4	GCTGGAAGTGGATTGTGG	CTTGTCGCTGTGAGGATAC	405	51
Hes1	GGCAGGCGCACCCCGCCTTG	GCAGCCAGGCTGGAGAGGCT	170	62
Hes5	ACCGCATCAACAGCAGCATT	AGGCTTTGCTGTGCTTCAGGT	135	55
Hey1	AAAGACGGAGAGGCATCATCG	GCAGTGTGCAGCATTTTCAGG	126	55
Jag1	AACTGGTACCGGTGCGAA	TGATGCAAGATCTCCCTGAAAC	216	54
Notch1	GCAGCCACAGAACTTACAAATCCAG	TAAATGCCTCTGGAATGTGGGTGAT	445	56
Notch2	GCTGTCCTCTTCATGCTGCA	AGCAGAAGTCAAGACAGTC	405	51
PCI	TGACCCCCAAAAGGACCAC	TGGTCCAGGTAGTAGGAATACCCA	237	54
Rhox5	TCATCATTGATCCTATTCAGGGTATG	CTCTCCAGCCTGGAAGAAAGC	380	55
Rn18s	CATTCGAACGTCTGCCCTAT	GTTTCTCAGGCTCCCTCTCC	128	56