Table 10. Comparison of the Prednisolone Active Ingredient Impurity Profile with the Certificate of Analysis and the Results Obtained with the Official Ph. Eur. Method.

method used	impurity F (%)	impurity B (%)	impurity A (%)	unknown (%)	unknown (%)	total impurities (%)
certificate of analysis obtained by the prednisolone producer	<LOQ	0.05	0.33	0.06	<LOQ	0.44
official Ph. Eur. (Table 1) (Venusil AQ C18; 150 × 4.6 mm, 3 μm)	<LOQ	0.06	0.36	0.06	<LOQ	0.48
developed gradient method (Table 5) (Gemini C18; 150 × 4.6 mm, 3 μm)	0.06	0.06	0.36	0.07	0.05	0.60