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| column | Gemini C18 (150 × 4.6 mm, 3 μm) |
|---|---|
| mobile phase | acetonitrile/tetrahydrofuran/water (20:10:70 v/v/v) |
| flow rate | 0.8 mL/min |
| injection volume | 10 μL |
| column temperature | 50 °C |
| detection | UV at 254 nm |
