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| column | Gemini C18 (150 × 4.6 mm, 3 μm) | ||
|---|---|---|---|
| mobile phase A | acetonitrile/tetrahydrofuran/water (15:10:75 v/v/v) | ||
| mobile phase B | 80% acetonitrile | ||
| gradient conditions | t (min) | mobile phase A (%) | mobile phase B (%) |
| 0 | 100 | 0 | |
| 9 | 90 | 10 | |
| 18 | 40 | 60 | |
| 20 | 100 | 0 | |
| flow rate | 0.8 mL/min | ||
| injection volume | 10 μL | ||
| column temperature | 50 °C | ||
| detection | UV at 254 nm | ||
