Figure 1.

Prediction of Malassezia furfur secreted proteases and assessment of extracellular protease activity in culture. (A) A comparison of the predicted secreted proteases in M. furfur CBS 14141 and M. globosa CBS 7966. (B) Unrooted phylogenetic tree of the predicted secretory aspartyl proteases in M. globosa (14, in black) and M. furfur (5, in red). Number at each node indicates the bootstrap value. (C) Extracellular protease activity of M. furfur cultured in 3 different media assessed using 19 internally quenched substrates. (D) Correlation of secreted protease activity using a representative substrate S9 with planktonic culture growth. Error bars represent standard deviation for n = 3.