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. 2020 Apr 9;10:148. doi: 10.3389/fcimb.2020.00148

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Copyright © 2020 Poh, Goh, Fan, Chua, Gan, Lim, Sharma, Leavesley, Dawson and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MfSAP1 on acute wound closure. (A) MTT staining (left) and hematoxylin and eosin (H&E, right) staining of the DED-HSE sections on Day 1 and Day 5. The dashed line indicates the edge of the punch biopsy. Black scale bar, 1 mm. (B) Quantification of the uncovered wound area as determined by MTT stain using ImageJ. Each wound area sample is normalized to the media control. Error bars represent standard deviation for n = 6 from a total of 3 experimental replicates with technical duplicates. (C) H&E and immunohistochemical analysis of the wound edge on Day 5 for each treatment condition. Representative images are shown from a total of 3 independent experiments. Red scale bar, 50 μm.