Fig. 4.
PI uptake of AMPs. S aureus JE 2 (A) and E. coli K12 (B) cells (1 × 107 CFU) were untreated, treated with AMPs (2xMIC) or treated with Polymixyn B (PMB, 16 μg/mL) for 120 min. The PI fluorescence intensity was then measured at 20 min intervals for a total of 120 min. PMB is known to kill Gram-negative bacteria by disrupting cell membrane and was used as a positive control.