Figure 2.

DHT-mediated ERβ suppression and BBR-mediated AR suppression by histone methylation on the promoters. (A) The conditional immortalized ACS-5003 neurons were transiently transfected by either ERβ full length (pERβ-2000) or deletion reporter plasmids. After 24 h, the cells were treated by either control (CTL) or 10 nM DHT for 24 h, and DHT-induced relative reporter activities (% control) were calculated, n = 4. *P < 0.05, vs. pERβ-2000 group. (B) The human ACS-5003 neurons were treated by CTL, 10 nM DHT (DHT), DHT with AR knockdown by lentivirus (DHT/shAR), or DHT with BBR (DHT/BBR) for 24 h, and cells were harvested for ChIP analysis, n = 4. *P < 0.05, vs. CTL group. (C) The conditional immortalized ACS-5003 neurons were transiently transfected by either AR full length (pAR-2000) or deletion reporter plasmids. After 24 h, the cells were treated by either CTL or 10 μM BBR for 24 h, and BBR-induced relative AR reporter activities (% control) were calculated, n = 4. *P < 0.05, vs. pAR-2000 group. (D) The human ACS-5003 neurons were treated as mentioned by (B), and then cells were harvested for ChIP analysis, n = 4. *P < 0.05, vs. CTL group. Data were expressed as mean ± SEM.