Figure 5.

Postnatal BBR treatment ameliorates prenatal DHT exposure-induced ALB in offspring, while postnatal shAR treatment does not affect. The 2-month female rats received either CTL or DHT treatment during their pregnancy, and the subsequent 2-month old male offspring from DHT group received either shAR lentivirus infusion in the amygdala (DHT/p-shAR) or DHT with BBR injection (DHT/p-BBR) for 1 week, and then the male offspring were used for ALB analysis followed by a biomedical analysis of the isolated amygdala. (A) The mRNA levels for gene expression of AR, ERβ and SOD2, n = 4. (B) Quantitation of protein levels, n = 5. (C) The representative pictures for western blotting for (B). (D) Ultrasonic vocalization, n = 9. (E) Social recognition, seconds spent socially investigating a conspecific [same conspecific in tests 1–4; novel conspecific in test 5 (a new stimulus rat was introduced)], n = 9. *P < 0.05, vs. CTL group; ^¶P < 0.05, vs. DHT group. (F,G) Three-chambered social tests, n = 8. (F) Time spent in the chamber for sociability. (G) Time spent in the chamber for social novelty. *P < 0.05, vs. CTL group. Data were expressed as mean ± SEM.