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. 2020 Apr 15;20:93. doi: 10.1186/s12866-020-01763-1

Fig. 4.

Fig. 4

Rv0148 interacts with Htdy. Pull-down assay confirms the interaction between Rv0148 and Htdy. The crude lysates enclosing His-Htdy and GST-Rv0148 were incubated with Ni-NTA resin; unbound proteins (lane 1) were washed off and incubated with their corresponding substrates. The protein fractions were eluted (lanes 2 and 4) and immunoblotted with anti-His and anti-GST antibodies. As a control, the bait proteins were incubated with bovine serum albumin protein (lane 3). Lane M, molecular mass standard