Fig. 2.

Chd9 upregulates RUNX2 expression in HEK293T cells. Expression profiles using ∆∆CT analysis [34] of exogenous Chd9 and endogenous downstream osteogenic genes, normalized against HPRT gene expression (HK). a Exogenous CHD9 expression levels. Exogenous Thr2384 and Ala2384 were strongly over-expressed compared to the empty vehicle, with greater expression in the Thr2384 transfected cells. b Endogenous osteogenic gene expression. Expression of Thr2384 and Ala2384 resulted in a significant increase in endogenous human RUNX2 expression, but not OC or ALP.c Relative RUNX2 expression. RUNX2 was compared against normalized exogenous Chd9 levels to determine the RUNX2 / CHD9 ratio. The Ala2384 allele resulted in a small but non-significant increase in endogenous RUNX2 expression compared to the Thr2384 allele