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Development of a D-alanyl-lipoteichoic acid biosynthesis protein (dltD) mutant from Lactobacillus plantarum CRL1506. (A) Summary of the strategy used for the development of L. plantarum ΔdltD by double-crossover. Primer sequences are listed in Table 1. (B) Confirmation of the deletion of 27 bp in the dltD gene by PCR analysis. (C) Growth curve and (D) electron microscope analysis of wild-type (WT) and ΔdltD mutant from Lactobacillus rhamnosus CRL1506.
