Table 2.
Overview of the mass spectrometry applications currently employed in plant hormone analysis
| Analytes | Analytical technique | Plant matrix | Recovery (%)a | LODs | Linearity | References |
|---|---|---|---|---|---|---|
| JA, ABA, SA, BA, GAs | LC–ESI–MS/MS | Hamlin trees leave (20 mg FW) | 34.6–50.3 | 0.03–3.00 ng mL−1 | 0.10–100.00 ng mL−1 | [75] |
| JAs, SA, ABA, IAA | UHPLC–ESI–MS/MS | A. thaliana leaf (20 mg FW) | – | 0.05–50 fmol | 0.05–500.00 pmol | [55] |
| IAA, GAs, tZ,ABA | LC–ESI-IT-MS/MS | A. thaliana (100 mg FW) | 70.0–100.0 | 0.55–170 fmol | 5.00–1000.00 fmol | [63] |
| BRs | UHPLC-MS/MS | Brassica napus (50 mg FW) | 30.9–88.9 | 1–50 pg | 0.01–10.00 pmol | [20] |
| K, iP, BA, ABA, NAA | HPLC–MS/MS | Vermicompost (250 mg) | 0.3–18.9 | 0.0015–0.3000 mg L−1 | 0.005–10.000 mg L−1 | [14] |
| IAA, ABA, GAs, SA, JA, tZ, 6-BA, iP | LC–ESI–MS/MS | Oilseed rape | 75.1–113.0 | 0.002–0.021 ng mL−1 | 0.0013–0.0210 ng mL−1 | [89] |
| tZ, K, KR | UHPLC–ESI–MS/MS | Tobacco (100 mg FW) | 68.8–103.0 | 2.4–47 pg mL−1 | 0.005–20.000 ng mL−1 | [90] |
| ABA, IAA, JA, SA, GAs, tZ | UHPLC–ESI–MS/MS | P. pinaster Aiton. clones needles (200 ± 20 mg DW) | 56.8–99.1 | 1.45–23.44 pg | 0.040–2500.000 ng mL−1 | [54] |
| BRs | UHPLC–ESI–MS/MS | A. thaliana (10 g FW) | 76.9–86.1 | 2.00–8.00 ng L−1 | 10.00–10,000.00 ng L−1 | [31] |
| GAs | UHPLC–ESI–MS/MS | Floral organs (about 80–250 μg) | 64.0–107.0 | Down to 5.41 amol | 0.01–25 fmol | [21] |
| IAA, ABA, JA, SA, IBA, GAs | CE-ESI-TOF–MS | Rice leaves (3 g) | 84.6–112.2 | 0.34–4.59 ng mL−1 | 1.30–850.00 ng mL−1 | [91] |
| IAA, ABA, JA, SA, IBA, GAs | nano-LC–ESI-QTOF-MS | Rice leaves (5 mg FW) | 88.3–104.3 | 1.05–122.4 pg mL−1 | 0.004–100.00 ng mL−1 | [52] |
| ABA, IAA, IBA, GAs, SA | HPLC-ESI-QTOF-MS | Green seaweeds (100 mg FW) | 80.0–92.0 | 0.5–1.0 μg mL−1 | 0.20–100.00 mg mL−1 | [92] |
aThe recovery is relative recovery