Fig. 2.

HG-induced proliferation and fibrosis in MCs were alleviated by PVT1 knockdown. a PVT1 expression by qRT-PCR in MCs transfected with si-NC, si-PVT1#1, and si-PVT1#2. MCs were exposed to 45 mM of HG or normal medium for 48 h, or transfected with si-NC or si-PVT1#1 before HG treatment, followed by the measurement of cell proliferation by MTS assay 0, 1, 2 and 3 days after transfection (b), Ki67 expression by western blot after 48 h transfection (c), the levels of α-SMA and FN by western blot 48 h after transfection (d). *P < 0.05