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PVT1 knockdown ameliorated HG-induced proliferation and fibrosis by miR-23b-3p/WT1 axis. MCs were transfected with si-PVT1#1 + anti-miR-NC, si-PVT1#1 + anti-miR-23b-3p, si-PVT1#1 + pcDNA, or si-PVT1#1 + WT1 overexpression plasmid before HG treatment, followed by the determination of cell proliferation by MTS assay (a), Ki67 expression by western blot (b), the levels of α-SMA and FN by western blot (c). pcDNA: nontarget negative plasmid, WT1: WT1 overexpression plasmid. *P < 0.05
