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. 2020 May;26(5):581–594. doi: 10.1261/rna.073965.119

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© 2020 Aguiar et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — EVE-derived piRNAs from the flamenco-like locus associate with mosquito PIWI proteins. (A) Size profile of small RNA derived from miRNAs or the flamenco-like locus in control or oxidation-treated libraries. The nt at the 5′ end is indicated by color. (B) Association of piRNAs derived from the top five uni- and dual-strand clusters (highlighted in Supplemental Table S4) compared to microRNAs with different PIWI proteins based on immunoprecipitations from mosquito Aag2 cells. The flamenco-like locus belongs to the group of uni-strand piRNA clusters and is highlighted in red for comparison. Statistics were performed using Wilcoxon rank-sum test. Significant P-values (P < 0.01) are indicated. Small RNA libraries from mosquito Aag2 cells were used in these analyses.