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. 2020 May;26(5):564–580. doi: 10.1261/rna.073577.119

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© 2020 Torgerson et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Glycine riboswitch types and scheme for gene regulation by a transcriptional ON tandem glycine riboswitch. Cartoon representation of the tandem (A), type-1 singlet (B), and type-2 singlet (C) variants of the glycine riboswitch. Two A-minor interactions (labeled as α and β) that occur between adjacent P1 and P3 helices are conserved among tandem systems. Singlet systems each retain one of these interactions. Glycine (orange) binds within the P3 helix of each aptamer domain. (D) Cartoon schematic of ligand-induced helical switching by a transcriptional ON tandem glycine riboswitch. Ligand binding stabilizes the P1 helix of aptamer 2, which acts as an antiterminator and promotes the production of full-length mRNA.