FIGURE 5.

Characterization of ligand response for WT and potential single-occupancy constructs. (A) Top-down view of the aptamer 1 binding pocket from the crystal structure of the F. nucleatum tandem glycine riboswitch (PDB ID: 3P49; Butler et al. 2011). Two hydrogen bonds are made between glycine and U81. (B) SMARTT-generated transcription termination response profiles for WT and all four uracil-to-purine binding site mutations. (C) Binding curves generated by equilibrium dialysis for WT and the binding site mutants U81G, U179G, and U81G/U179G. Error bars represent the standard deviation across three replicates at each data point.