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Example of Experimental Design
| Number of animals euthanized/sex |
||||||||
|---|---|---|---|---|---|---|---|---|
| Group number | Identity of group | Total dose volume (mL) | Dose concentration (μg/ml) | Number of animals/sex | Core study animals |
Recovery animals |
||
| Day | Day | Day | Day | |||||
| 1 | vehicle | 0.5 | Same as clinical | 5 rabbits | ||||
| 2 | adjuvant(s) + vehicle | 0.5 | Same as clinical | 5 rabbits | ||||
| 3 | antigen + vehicle | 0.5 | Same as clinical | 5 rabbits | ||||
| 4 | antigen + adjuvant(s) + vehicle | 0.5 | Same as clinical | 5 rabbits | ||||
