TABLE 2.
Bacterial strains and plasmids used in this study.
Strain or plasmid | Description | Reference |
Escherichia coli DH5α | General cloning host, F-thi-1 endA1 hsdR17 (r-,m-) supE44 _lacU169 (_80lacZ_M15) recA1 gyrA96 relA1 | Stratagene |
Bacillus methanolicus | ||
MGA3 | Wild type strain | ATCC 53907 |
PB1 | Wild type strain | ATCC 51375 |
BVD | Wild type strain | Brautaset et al., 2004 |
RCP | Wild type strain | Brautaset et al., 2004 |
HEN9 | Wild type strain | Brautaset et al., 2004 |
SC6 | Wild type strain | Brautaset et al., 2004 |
DFS2 | Wild type strain | Brautaset et al., 2004 |
CFS | Wild type strain | Brautaset et al., 2004 |
JCP | Wild type strain | Brautaset et al., 2004 |
N2 | Wild type strain | Brautaset et al., 2004 |
NIWA | Wild type strain | Brautaset et al., 2004 |
TSL32 | Wild type strain | Brautaset et al., 2004 |
Plasmid | ||
pHP13 | CmR and EmR, E. coli/Bacillus spp. shuttle vector | DSM 8773 (Haima et al., 1987) |
pBV2mp-cadA | KmR and ApR; pBV2mp derivative for cadA expression under control of the mdh promoter | Irla et al., 2016a |
pTH1mp-gadst | CmR; pTH1mp derivative for gad from Sulfobacillus thermosulfidooxidans DSM 9293 expression under control of the mdh promoter | Irla et al., 2016b |
pTH1mp-lysC | pHP13 derivate with lysC under control of mdh promoter | Brautaset et al., 2010 |
pHYcrtMN | B. subtilis and E. coli shuttle vector; ori-pACYC177; ori-pAMα1; crtM and crtN genes of S. aureus; AmpR; TcR | Yoshida et al., 2009 |
pTH1mp-crtMN | pHP13 derivative carrying crtMN from S. aureus under control of the mdh promoter | This study |