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. 2020 Apr 9;11:680. doi: 10.3389/fmicb.2020.00680

TABLE 2.

Bacterial strains and plasmids used in this study.

Strain or plasmid Description Reference
Escherichia coli DH5α General cloning host, F-thi-1 endA1 hsdR17 (r-,m-) supE44 _lacU169 (_80lacZ_M15) recA1 gyrA96 relA1 Stratagene
Bacillus methanolicus
MGA3 Wild type strain ATCC 53907
PB1 Wild type strain ATCC 51375
BVD Wild type strain Brautaset et al., 2004
RCP Wild type strain Brautaset et al., 2004
HEN9 Wild type strain Brautaset et al., 2004
SC6 Wild type strain Brautaset et al., 2004
DFS2 Wild type strain Brautaset et al., 2004
CFS Wild type strain Brautaset et al., 2004
JCP Wild type strain Brautaset et al., 2004
N2 Wild type strain Brautaset et al., 2004
NIWA Wild type strain Brautaset et al., 2004
TSL32 Wild type strain Brautaset et al., 2004
Plasmid
pHP13 CmR and EmR, E. coli/Bacillus spp. shuttle vector DSM 8773 (Haima et al., 1987)
pBV2mp-cadA KmR and ApR; pBV2mp derivative for cadA expression under control of the mdh promoter Irla et al., 2016a
pTH1mp-gadst CmR; pTH1mp derivative for gad from Sulfobacillus thermosulfidooxidans DSM 9293 expression under control of the mdh promoter Irla et al., 2016b
pTH1mp-lysC pHP13 derivate with lysC under control of mdh promoter Brautaset et al., 2010
pHYcrtMN B. subtilis and E. coli shuttle vector; ori-pACYC177; ori-pAMα1; crtM and crtN genes of S. aureus; AmpR; TcR Yoshida et al., 2009
pTH1mp-crtMN pHP13 derivative carrying crtMN from S. aureus under control of the mdh promoter This study