Osteoinductive efficiency of supramolecular hydrogels. (A) Fluorescence intensity of alamarBlue towards MSCs after incubation in culture medium for 1, 3 and 7 days (n=3). (B) ALP activity (n=3, *p < 0.05, **p < 0.001 by one-way analysis of variance, ANOVA) and (C) ALP expression of cell-encapsulated hydrogels with LNSs ranging from 2.5 to 4.5 wt % treated in osteogenic medium for 1, 3, 7 and 14 days. Scar bar repents 1 mm. (D) Mineralization of cell-encapsulated hydrogels with LNSs ranging from 2.5 to 4.5 wt % after incubation with osteogenic medium for 3, 7, 14 and 21 days. Scar bar repents 1 mm.