Concentration‐dependent effects of local administration of RJR2403 into the M2 on releases of GABA and L‐glutamate in the secondary motor cortex (M2; Study 2). Concentration‐dependent effects of perfusion with α4β2‐nAChR agonist, RJR2403 (10, 30 and 100 μM) into the M2 on releases of GABA (a, b) and L‐glutamate (d, e) in the M2 of wild type (a, d) and S286L‐TG (b, e). Ordinates indicate mean extracellular levels of GABA and L‐glutamate (μM; N = 6), and abscissas indicate time after RJR2403 administration (min). Grey bars indicate the perfusion with RJR2403 into the M2. (c, f) The AUC value of extracellular levels of GABA and L‐glutamate (nmol) during perfusion with RJR2403 (from 20 to 120 min) in panels (a), (b), (d) and (e), respectively. Grey columns in panels (c) and (f) indicate the AUC values of basal extracellular levels of GABA and L‐glutamate in panels (a), (b), (d) and (e). *P < .05 relative to control and #
P < .05 relative to wild type by multivariate ANOVA with Tukey's post hoc test