Table 1.

Probes and primers used for qRT‐PCR analysis

Gene	Probes	Forward primer	Reverse primer
Wild Chrna4	5′‐TAGCCAATATCTCAGATGTGGTCCTCGT‐3′	5′‐CAGCCACATAGAGACCCG‐3′	5′‐GAGCAATGGACAAGCCAAAG‐3′
S286L Chrna4	5′‐AATAAATGGTCCCGCCCCGTGG‐3′	5′‐GCTCAAAAGGCTGTTTTCTGG‐3′	5′‐ATAGATAATCCGAACCGCACC‐3′
Total Chrna4	5′‐ACCCCCAGCCATCTA‐3′	5′‐CTATGACGGAAGGGTGCAGTG‐3′	5′‐ GTCGATGCTGCAGGAGCTCT‐3′