Table 6.
Effect of BIA 10‐2474 treatment (10, 30, or 100 mg·kg−1 p.o.) for 1 to 28 days on serine hydrolase enzymes in rat brain
| Serine hydrolase | Protein ID | BIA 10‐2474 treatment condition (mg·kg−1·day−1 p.o.) | Protein ID and prevalence in controls | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10 mg·kg−1 | 30 mg·kg−1 | 100 mg·kg−1 | Sequences for ID | Log2 intensity | ||||||||||||
| 1 day | 7 days | 28 days | 1 day | 7 days | 28 days | 1 day | 7 days | 28 days | 10 | 30 | 100 | 10 | 30 | 100 | ||
| FAAH1 | P97612 | 98 | 98 | 98 | 99 | 99 | 99 | 99 | 99 | 99 | 31 | 27 | 30 | 30.8 | 28.7 | 27.7 |
| Monoacylglycerol lipase (ABHD6) | Q5XI64 | 85 | 81 | 83 | 88 | 82 | 91 | 95 | 93 | 95 | 22 | 20 | 21 | 30.5 | 28.0 | 27.3 |
| Carboxyl esterase 1C | P10959 | 47 | 57 | 66 | 79 | 71 | 90 | 97 | 95 | 100 | 22 | 20 | 21 | 29.8 | 27.5 | 26.7 |
| Carboxyl ester hydrolase | D3ZGK7 | 69 | 73 | 79 | 98 | 67 | 99 | 99 | 100 | 100 | 3 | 3 | 3 | 24.9 | 22.9 | 22.9 |
| Carboxyl ester hydrolase | M0R9W7 | 12 | 25 | 12 | 9 | 71 | 94 | 81 | 97 | 98 | 9 | 6 | 7 | 24.5 | 21.9 | 21.4 |
| ABHD11 | D3ZXK4 | 3 | — | 11 | 10 | — | 39 | 64 | 64 | 82 | 16 | 13 | 14 | 30.9 | 28.6 | 27.9 |
| Signal peptidase complex catalytic subunit SEC11/11A | P42667; Q6P9X2 | 5 | 11 | 80 | 17 | 92 | 94 | — | — | — | 4 | 3 | 2 | 23.8 | 22.4 | 17.0 |
| Lysosomal thioesterase PPT2 | O70489 | — | — | 19 | 64 | 71 | 94 | nd | nd | nd | 5 | 3 | nd | 22.4 | 21.6 | nd |
| PLA2 Group 15 (PLA2G15) | Q675A5 | — | — | 24 | — | — | 43 | 29 | 58 | 71 | 9 | 10 | 8 | 25.6 | 23.3 | 22.5 |
| Patatin‐like phospholipase domain containing 6 (PNPLA6) | M0R715 | — | — | 12 | — | — | 30 | 17 | 44 | 63 | 61 | 47 | 46 | 29.1 | 26.6 | 25.8 |
| ABHD10 | Q5I0K5 | — | — | 6 | — | — | 12 | 9 | 38 | 63 | 18 | 16 | 16 | 30.7 | 28.1 | 27.3 |
| CaI‐PLA2 (PLA2G6) | P97570 | — | — | — | — | — | 42 | 7 | 81 | 69 | 9 | 6 | 4 | 22.7 | 20.2 | 19.7 |
| AIG1 protein | B2RZC0 | — | 9 | 9 | 11 | 10 | 53 | 19 | 62 | 72 | 6 | 6 | 6 | 29.6 | 26.2 | 26.7 |
Note. Values shown are % reduction of FP probe‐labelled serine hydrolase compared with vehicle as identified and quantified by LC/MS/MS. Each value is derived from six independent evaluations. Shaded cells with values in bold indicate proteins harbouring a fold change <−2 and an adjusted P value <.05 (Limma test and Benjamini–Hochberg correction for multiple testing). The experiment was analysed in three separate sub‐experiments, one for each of the dose levels. The protein ID and prevalence columns indicate the number of unique sequences used for identification of the protein in each sub‐experiment, and the log2 intensity of the protein by LC/MS/MS is given for the control group during each sub‐experiment. No value indicates no observed decrease in fold change.
Abbreviation: nd, not detected.