Figure 9.

MCF7 cells resistant to 4xAC migrated faster, formed larger number of spheroids, and overexpressed adhesion molecules when compared with resistant variants to 4xAC + 4xPAC. (a) Wound healing assay showed a significant reduction in the migration ability of cells resistant to 4xAC + 4xPAC when compared with control (n = 5) and cells resistant to 4xAC (n = 5). The graph represents the migration distance measured in micrometres, scale bar = 2,000 μm. (b) Spheroids formed by MCF7 control and resistant cells have different pattern in terms of density and number formed. MCF7 resistant to 4xAC + 4xPAC formed the densest spheroid, scale bar = 2,000 μm. (c) Resistant MCF7 cells exhibited increased adhesion mediators such as ZO1, claudin‐1, N‐cadherin, and E‐cadherin that were significantly increased in MCF7 cells resistant to 4xAC and 4xAC + 4xPAC. All experimental data are from five independent experiments (n = 5). *P < .05 significantly different from control cells; ANOVA and Tukey's post hoc test