Figure 2.

Melatonin transformed the cytokine framework in osteoporosis mice. (a) Gene presentations of osteogenic markers in bone tissue were detected. (b) Immunohistopathology (200×) showed the protein presentations of matricellular molecular osteocalcin (OCN) and osteogenic marker Osterix in proximal tibias bone tissue. (c–f) The concentrations of bone resorption markers CTX1 (c) and TRAP (d), osteoclast maturation‐related cytokine RANKL (e), and inflammation cytokine TNF‐α (f) in serum were determined by ELISA. Data are expressed as the mean ± SD, and n = 6 in each group; *P < .05, significant differences between each indicated group. NS, not significant