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. 2020 Apr 7;11(1):315–326. doi: 10.1080/21505594.2020.1748923

Figure 6.

Figure 6.

(a) Detail of a selected conserved region in the predicted secondary structure of the S. enterica CheW and A. baumannii CheW-like proteins. The large and small green boxes indicate, respectively, a known domain and a residue of S. enterica CheW protein involved in the interaction with RecA. The black circles indicate the subsequently mutagenized residues from the A. baumannii CheW-like protein. (b) Co-immunoprecipitation of the RecA-FLAG and A1S_2813-6× His proteins and the derivative A1S_2813-6× His site-specific mutagenized proteins. The supernatants were separated by SDS-PAGE and assessed by western blotting. The images are representative of those from three independent assays. The presence of the RecA-FLAG and A1S_2813-6× His proteins (WT) and the absence of (-) or residue change (Ser97Ala or Ile121Ala) in A1S_2813-6× His in the corresponding mixtures are indicated. The western blot shows the RecA-FLAG and A1S_2813-6× His protein bands revealed following incubation of the lysates with anti-FLAG coated beads. M: molecular mass marker.