Table 3.

Permeability results (P_e, 10⁻⁶ cm/s) from the PAMPA–BBB assay for compounds 12, 20–24, 30–38, and 52 and their prediction of BBB penetration.

Compd.	Pe (10−6 cm/s)a	Predictionb
NTZ	1.26±0.21	CNS+/−
12	5.76±0.57	CNS+
20	0.45±0.03	CNS−
21	0.09±0.01	CNS−
22	6.02±0.27	CNS+
23	4.92±0.31	CNS+
24	12.62±1.22	CNS+
30	5.76±0.55	CNS+
31	3.42±0.07	CNS+
32	6.16±0.50	CNS+
33	10.41±1.25	CNS+
34	6.82±0.50	CNS+
35	9.24±0.47	CNS+
36	10.62±0.10	CNS+
37	9.89±0.37	CNS+
38	9.88±0.75	CNS+
52	10.50±1.13	CNS+

^aValues are expressed as the mean ± SD (n ≥ 3).

^bCompounds with permeabilities P_e > 3.08 × 10⁻⁶ cm/s could cross the BBB by passive diffusion (CNS+). Compounds with P_e < 1.13 × 10⁻⁶ cm/s show low BBB permeation (CNS−), and compounds with 1.13 × 10⁻⁶ cm/s < P_e < 3.08 × 10⁻⁶ cm/s show uncertain BBB permeation (CNS+/−).