KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Sheep polyclonal anti-GFP | Bio-Rad | Cat#4745-1051; RRID: AB_619712 |
| Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) | Invitrogen | Cat#A-11015; RRID: AB_141362 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAPI fluorescent DNA stain | ThermoFisher Scientific | Cat#D1306 RRID: AB_2629482 |
| Critical Commercial Assays | ||
| Protein G Dynabeads for immunoprecipitation | Invitrogen | Cat#10003D |
| Proteinase K | NEB | Cat# P8107S |
| NEBNext Ultra II DNA Library Prep Kit for Illumina | NEB | Cat#E7645S |
| NEBNext Multiplex Oligos for Illumina Kit | NEB | Cat#E7335S |
| Zymogen ChIP DNA Clean and Concentrator Kit | Zymogen | Cat#D5205 |
| PowerUp SYBR Green Master Mix | Applied Biosystems | Cat#A25741 |
| Deposited Data | ||
| Raw ChIP-seq data and list of Cic targets | This paper | GEO: GSE130584 |
| Analysis scripts | This paper | https://github.com/keenansh/SEK_Cic_ChIP_Analysis. |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster:–_OregonR | Bloomington Drosophila Stock Center | Stock# 5 RRID:BDSC_5 |
| D. melanogaster:–_UAS-OptoSOS | (Johnson et al., 2017) | N/A |
| D. melanogaster:–_CicsfGFP | This paper | N/A |
| D. melanogaster:–_orD4021 | (Schüpbach and Wieschaus, 1986) | FlyBase: FBal0016921 |
| D. melanogaster:–_MTD-Gal4 (Maternal Triple Driver) | Bloomington Drosophila Stock Center | Stock# 31777 RRID:BDSC_31777 |
| D. melanogaster: tll-MS2 | This paper | N/A |
| D. melanogaster: MCP-mCherry | Levine Lab | N/A |
| Oligonucleotides | ||
| Forward primer for qPCR at tll enhancer, 5′ GGGTGGTTACCTGGCTTAGG 3′ | This paper | N/A |
| Reverse primer for qPCR at tll enhancer 5′ ACGCCGTTCAGTCGCTAATC 3′ | This paper | N/A |
| Software and Algorithms | ||
| Fiji | (Schindelin et al., 2009) | RRID: SCR_002285 |
| SAMtools | (Li et al., 2009) | RRID:SCR_002105 |
| MACS2 | (Zhang et al., 2008) | RRID:SCR_013291 |
| PicardMarkDuplicates | (http://broadinstitute.github.io/picard/) | RRID: SCR_006525 |
| RStudio | https://RStudio.com | RRID:SCR_000432 |
| R package: GenomicRanges | (Lawrence et al., 2013) | RRID:SCR_000025 |
| R package: ChIPSeeker | (Yu et al., 2015) | https://www.bioconductor.org/packages/release/bioc/html/ChIPseeker.html |
| Galaxy | https://usegalaxy.org | RRID:SCR_006281 |