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Purification of wild‐type and mutant VP1 protein and replicase activity. (A) SDS‐PAGE of purified wild‐type His‐VP1 and His‐VP1 DD764–765AA mutant. Lanes 1 and 3, protein size markers (kDa); lane 2, VP1 mutant; lane 4, wild‐type VP1. (B) Replicase assay of wild‐type and mutant DD764–765AA VP1 proteins. Lane 1 32P‐labeled BTV‐10 genomic dsRNA; lane 2, replicase assay products using His‐VP1 DD764–765AA; lane 3, replicase assay products using wild‐type His‐VP1.
