Figure 4.

Thermal stability of the surface coating and the immobilized PCR amplicons under the influence of thermal cycling condition. The fluorescence intensities of the immobilized amplicons were compared before and after the thermal treatment. Data are presented as mean ± standard deviation (n = 3). (a) Schematic of PEI‐GA‐coated PC immobilized with amplicons and (c) the corresponding fluorescence image result. (b) The schematic of the thermal treatment and (d) the corresponding fluorescence image result. The amplicons were amplified off‐chip from 10 ng (3.25 × 10⁶ copies) DNA template. GA, glutaraldehyde; PC, polycarbonate; PEI, polyethyleneimine; PCR, polymerase chain reaction [Color figure can be viewed at wileyonlinelibrary.com]