Table Table 4.
Purification Data from Lee and Sagar (70)
| stage | plasmid product (mg) | % step yield | genomic DNA (mg/mg) | protein (mg/mg) | RNA (mg/mg) | LAL (EU/mg) |
|---|---|---|---|---|---|---|
| clarified lysate a | 6750 | 100 | 0.52 | 7.6 | 196 | 1.1 × 107 |
| concentration/RNase b /diafiltration/dead‐end filtration | 6500 | 93 | 0.50 | 1.6 | 2.21 | 3.4 × 106 |
| anion exchange c | 4000 of 5000 | 80 | 0.41 | 0.3 | 0.1 | 1.2 × 104 |
| reversed phase d | 2300 of 3200 | 77 | 0.029 | <0.01 f | <0.01 f | 62 |
| concentration/diafiltration into final buffer | 2110 | 100 | 0.029 | <0.01 f | <0.01 f | 2.8 |
| final process yield e | 54 |
a The original lysate was 4.5 L of paste in 33.7 L. b Ribonuclease was used. c The anion exchanger (POROS PI/M) would have a volume of 80 L based on 1 m3 of unclarified lysate. d The revered phase medium (POROS R2/M) would have a volume of 154 L based on 1 m3 of unclarified lysate. e The yield from 1 m3 of clarified lysate would be 95 g. The contaminants are given as mg per mg plasmid DNA. f Below detection limits of assay method.