Table 2.
Examples of genetic manipulation in cell line development for vaccine manufacture
| Cell origin | Cell line name | Type of vaccines | Genetic manipulation in cell line development | Ref. |
|---|---|---|---|---|
| HER (Human embryonic retinoblasts) | HER.911 | Designed for vectored vaccines (and viral vectors) based on E1‐deleted replication deficient human adenovirus type 5 | Expression of the Ad serotype 5 (Ad5) sequence (nucleotides 79 – 5789) | [99] |
| HER | PER.C6 | Constitutive expression of Ad5 E1A‐ and E1B‐encoding sequences (nucleotides 459–3510) under the control of the human phosphoglycerate kinase (PGK) promoter. | [132] | |
| Primary human amniocytes | N52.E6 | Constitutive expression of Ad5 E1A‐ and E1B‐encoding sequences (nucleotides 505–3522) under the control of the mouse PGK promoter | [101] | |
| HeLa | GH329 | Constitutive expression of Ad5 E1A‐ and E1B‐encoding sequences (nucleotides 511–3924) under the control of the mouse PGK promoter | [100] | |
| Primary muscovy | AGE1.CR | Propagation of attenuated MVA | Constitutive expression of human Ad5 E1A | [105] |
| duck cells | AGE1.CA | under control of the human PGK promoter and | ||
| (retina (CR), somite (CS) and amnion membrane (CA)) | AGE1.CS | Propagation of other viruses has also been demonstrated | bovine growth hormone polyadenylation site and E1B is under control of herpes simplex virus thymidine kinase (HSV‐TK) promoter and polyadenylation site. | |
| CHO (Chinese hamster ovary cells) | CHO‐HBsAg | Hepatitis B surface antigen HBsAg | Constitutive expression of the HBsAg genes selected under methotrexate | [106] |
| HEK 293 (Human embryonic kidney cells) | HEK‐G | VLP‐based vaccine for rabies | Constitutive expression of the rabies virus G protein for continuous production of rabies virus VLPs | [107] |
| RK13 (Rabbit kidney cells) | J12#26 | VLP‐based vaccine for Japanese encephalitis (JEV) | centerConstitutive expression of C , prM, and E proteins from JEV, strain Beijing‐1 | [133] |
| BHK‐21 (Hamster kidney cells) | BJ‐ME | Constitutive expression of codon‐optimized cDNA encoding JEV prM and E protein, strain SA14‐14‐2 | [108] | |
| MDCK (Canine kidney cells) | MDCK SFS | Attenuated influenza vaccine (attenuated by NS1 deletion) | Establishment of two stable MDCK cell lines that show inducible expression of the allele B NS1 protein | [134] |