Table 6.

Summary of methods for antimicrobial liposome preparation

Method	Formulation	Solvent	Size	Ref.
Dehydration‐rehydration vesicles	Egg phosphatidylcholine,78 phosphatidic acid (PA), stearylamine (SA), distearoyl phosphatidylcholine (DSPC), cholesterol	–	–	117
Reverse‐phase evaporation vesicles	Dipalmitoyl‐DL‐α‐phospatidyl‐l‐serine (PS), cholesterol, egg phosphatidylcholine (EPC), 1,2‐dipalmitoyl‐sn‐glycerophosphocholine monohydrate, 1,2‐dimyrostoyl‐sn‐glycerophosphoethanolamine (PE),1,2‐dipalmitoyl‐sn‐glycerophosphatidic acid disodium salt (PA), dihexadecyl hydrogen phosphate (DP)	Diethyl ether, isotonic buffer	207–265	118
Freeze‐thaw multilamellar vesicles	Dipalmitoyl‐DL‐α‐phospatidyl‐l‐serine (PS), cholesterol, egg phosphatidylcholine (EPC), 1,2‐dipalmitoyl‐sn‐glycerophosphocholine monohydrate, 1,2‐dimyrostoyl‐sn‐glycerophosphoethanolamine (PE), 1,2‐dipalmitoyl‐sn‐glycerophosphatidic acid disodium salt (PA), dihexadecyl hydrogen phosphate (DP)	Phosphate‐buffered saline (PBS)	945–1307	78
Stable plurilamellar vesicles	Egg phosphatidylcholine, CHL, dicetylphosphate (DP), stearylamine (ST), bovine HDL	Diethyl ether, HEPES buffer	–	119
Electrospray	Lecithin	Polyethylene glycol, poly(allylamine hydrochloride)	1300–4600	120

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