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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Anal Chem. 2020 Mar 5;92(6):4436–4444. doi: 10.1021/acs.analchem.9b05388

Figure 5. Sequence coverage and product ion distributions following dissociation of the 21-nucleotide RNA strand via HCD, CAD, NETD, and AI-NETD.

Figure 5.

(A) The percent sequence coverage is shown for electron- and collision-based dissociation methods. Grey bars indicate when all eight fragment types were considered, whereas a dashed orange line indicates integration of only d- and w-type ion data, the main products of NETD approaches. (B) The undesirable internal fragments are monitored as a function of a percentage of the total ion current (% TIC) for each individual experiment. (C) The overall product ion distribution, based on relative intensity, for all eight fragment types and base-loss fragments.