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. 2020 Apr 16;15(4):e0231634. doi: 10.1371/journal.pone.0231634

Fig 3. HUVECs WST-1 activity is dose-dependently reduced by direct exposure to TWIP steel particles.

Fig 3

HUVECs were grown in 96-well transparent or white plates and exposed the next day to different concentrations of TWIP steel or 316L steel particles or extracts for 24h. Extracts were obtained from culture medium incubated during 24h with the same concentrations of particles. The cells were then washed and further incubated in fresh medium with 10% WST-1 reagent for 2h. Absorbance was measured at 450 nm, with 690 nm as reference (A, C). After 24h exposure and wash, the white plate was replenished with fresh medium containing the CellTiter-Glo® reagent (ATP) and luminescence was read (B, D). Results are reported as relative WST-1 activity or luminescence, where 1.0 corresponds to the value measured in control cultures. Min-U-Sil® was used as a positive control. Values are means ± SEM (n = 8), *p < 0.05 relative to control (One-way ANOVA followed by a Dunnett’s multiple comparison). The trend test included controls.