. 2020 Apr 16;15(4):e0231673. doi: 10.1371/journal.pone.0231673

Table 2. The peak areas × 10⁻⁵ (mean ± S.E.) and descriptions of odor-active volatile organic compounds in Mesocentrotus nudus gonads of each group (n = 3).

			Peak area (×10⁻⁵)				Description
NO.	Rt	Compound name	Cultured	Eisenia	Barren	p	Cultured	Eisenia	Barren
		Alcohols
11	13.21	2-propanol	6.94 ± 1.09	8.25 ± 1.27	10.29 ± 0.61	0.076	Kelp, seaweed, hey smell
12	13.46	Ethanol	456.02 ± 212.64	559.31 ± 221.89	503.28 ± 187.53	0.922		Mint
20	16.57	2-butanol	1.72 ± 0.39	1.70 ± 0.41	2.80 ± 0.52	0.216	Seafood, hey smell	Unpleasant, sea urchin
45	30.41	2-ethylhexanol	616.30 ± 284.38	526.13 ± 52.00	566.66 ± 102.69	0.938	Sea urchin, seafood	Sea urchin
		Aldehydes
1	5.87	Acetaldehyde	6.12 ± 1.93	3.95 ± 1.24	3.72 ± 0.75	0.392		Putrid
2	6.74	Dimethoxymethane	3.12 ± 0.54	2.65 ± 0.35	2.65 ± 0.65	0.755	Green, acrid	Putrid
7	11.78	Acetal	1.77 ± 1.33	2.31 ± 2.57	0.93 ± 0.32	0.714	Sweet
33	21.97	2-ethylhexanal	0.49 ± 0.09	0.21 ± 0.18	0.36 ± 0.01	0.119	Sweet
39	25.10	Octanal	0.15 ± 0.01	0.14 ± 0.04	0.19 ± 0.02	0.193	Green	Putrid	Green
42	28.12	Nonanal	2.31 ± 0.16	2.54 ± 0.54	2.60 ± 0.29	0.723	Plastic, medicinal
46	30.92	Decanal	2.74 ± 0.39	2.51 ± 0.51	2.45 ± 0.29	0.813			Green
47	32.01	Benzaldehyde	0.22 ± 0.06	0.21 ± 0.05	0.23 ± 0.02	0.962	Sea urchin
		Aromatic compounds
4	7.79	Methylcyclohexane	3.29 ± 0.27	2.74 ± 0.89	4.05 ± 0.39	0.150	Benzene
13	13.63	Benzene	0.62 ± 0.05	0.62 ± 0.17	0.66 ± 0.15	0.958	Tingle in nose, heave note	Mint
23	17.26	Toluene	71.64 ± 16.58	54.25 ± 17.59	59.44 ± 7.81	0.606	Fruit	Sweet
27	19.87	Ethylbenzene	54.44 ± 10.42	61.95 ± 9.12	58.98 ± 10.80	0.873	Sea urchin, baked fish	Putrid fish, sour
36	23.77	Trimethylbenzene	1.03 ± 0.09	1.26 ± 0.41	1.07 ± 0.06	0.542	Hey smell
37	24.23	Styrene	0.68 ± 0.12^b	0.86 ± 0.16^b	1.91 ± 0.08^a	< 0.001	Hey smell	Hey smell
44	29.74	Dichlorobenzene	4.43 ± 1.32	3.83 ± 1.50	6.62 ± 0.93	0.227	Green
		Esters
6	11.56	Ethyl acetate	11.76 ± 1.40	10.51 ± 1.28	8.65 ± 0.75	0.171	Sweet
14	14.80	Propyl acetate	3.00 ± 0.25	2.74 ± 1.47	2.33 ± 0.24	0.616	Sweet, sweet candy
24	17.48	S-methyl thioacetate	10.75 ± 9.61^a	0.38 ± 0.33^a	ND^b	< 0.001	Tingle in nose, heavy note, fishy
25	18.18	Butyl acetate	0.73 ± 0.13	0.62 ± 0.11	0.48 ± 0.12	0.327	Green	Putrid
		Halomethanes
19	16.26	Chloroform	8.17 ± 1.82	5.61 ± 1.01	6.55 ± 1.94	0.542	Green, hey smell		Plastic, medicinal
43	29.63	Bromoform	0.12 ± 0.04^a	0.04 ± 0.01^b	ND^c	< 0.001	Green
		Hydrocarbons
16	15.53	Decane	2.02 ± 0.41	2.56 ± 1.47	2.32 ± 0.53	0.833		Fishy
26	18.56	Undecane	2.25 ± 0.17	2.10 ± 0.57	2.67 ± 0.50	0.545	Tingle in nose, heavy note	Oxidized seaweed, hey smell
		Ketones
5	9.12	Acetone	116.18 ± 45.05	103.60 ± 20.71	113.94 ± 9.31	0.926			Sweet
9	12.13	2-butanone	11.62 ± 2.92	9.41 ± 3.49	7.85 ± 1.45	0.521	Peach
41	26.59	6-methyl-5-hepten-2-one	2.61 ± 0.72	1.87 ± 1.13	2.29 ± 0.70	0.761		Sweet, citrus fruit
		Others
3	7.09	Dimethyl sulfide	16.18 ± 7.39	9.47 ± 1.59	65.17 ± 48.88	0.261			Putrid
17	15.72	Acetonitrile	48.98 ± 20.79	44.59 ± 18.81	56.33 ± 17.72	0.887			Tree bark
40	25.42	Bis-(methylthio)-methane	2.32 ± 0.96	1.92 ± 0.92	0.97 ± 0.11	0.257	Fishy

Significance of values in peak areas among groups analyzed by ANOVA are provided. ND indicate no detection. An explanation of the terms Cultured, Eisenia and Barren is provided in Fig 1. Lower-case letters indicate significant differences in peak areas among groups (p < 0.05, Tukey’s test). An explanation of the term Rt is provided with Table 1.

Table 2. The peak areas × 10−5 (mean ± S.E.) and descriptions of odor-active volatile organic compounds in Mesocentrotus nudus gonads of each group (n = 3).

Table 2. The peak areas × 10⁻⁵ (mean ± S.E.) and descriptions of odor-active volatile organic compounds in Mesocentrotus nudus gonads of each group (n = 3).